An accurate, simple, reproducible, and sensitive HPLC method was developed and validated for the estimation of Propranolol hydrochloride (PRH) and Flunarizine dihydrochloride (FLZ) in solid dosage form. The analyses were performed on a kromasil C18 column, 250 × 4.60 mm id, 5 μm particle size. The mobile phase methanol: buffer (20 mM phosphate buffer, pH-3): TEA (80: 20: 0.4 v/v/v), was pumped at a constant flow rate of 1.0 mL/min. UV detection was performed at 240 nm. Retention times of Propranolol hydrochloride and Flunarizine dihydrochloride were found to be 2.90 and 4.10 min, respectively. The LOD & LOQ is 1.09ug/ml & 3.33ug/ml for PRH & 0.34ug/ml & 1.02ug/ml for FLZ. The method was validated in terms of linearity, precision, accuracy, LOD, LOQ, and robustness. The response was linear in the range 10-50 μg/mL (r2 = 0.995) for Propranolol hydrochloride, 2.5-12.5 μg/mL (r2 = 0.996) for Flunarizine dihydrochloride.
Loading....